CV

Education

M.S. in Plant Biology with specialization in Ecology, Evolution, and Behavior, Michigan State University, 2022
B.S. in Clinical Plant Science, Hosei University, 2019

Work experience

May 2023 - Present: Laboratory Technologists
- Vetrinary DIagnostic Laboratory, Michigan State University
- Duties includes: molecular Diagnostic, development of bioinformatics pipeline
January 2022 - May 2023: Instructor
- Biological Sciences Program, Michigan State University
- Duties included: Lectures, demostration of molecular biology techniques, faciltate student-led research

Skills

Bioinformatics
- Language: R, Unix, Python
- Operation and development of reproducible and scalable bioinformatics pipeline on HPCC
  - Tools: Nextflow, snakemake, git, conda, “renv” package
- Experiences in viral metagenomic analysis, phylogenetic analysis, variant calling analysis
- GUI tools: Geneious, CLC genomics workbench,
Molecular Biology
- DNA/RNA extraction, PCR, RT-PCR, Agarose gel electrophoresis, Sanger sequencing, QIAxcel operation
- Library preparation for Illumina-based sequencing platform
Plant Virology
- Maintanance of aphid culture, aphid-borne viruses, and plants
- Plant virus inoculation with plant sap, insect, and grafting
- Viral particule purification

Publications

First Report of Tobacco Ringspot Virus Infecting Pawpaw Orchard (Asimina triloba (L.) Dunal) in North America.

Maclot FJ, Mandujano M, Nakasato K, Byrne J, Paudel S, Guyer D, Malmstrom C. First Report of Tobacco Ringspot Virus Infecting Pawpaw Orchard (Asimina triloba (L.) Dunal) in North America. Plant Dis. 2022 Dec 26. doi: 10.1094/PDIS-11-22-2639-PDN. Epub ahead of print. PMID: 36572972.

First detection of two potyviruses, uraria mosaic virus and passiflora foetida virus Y, from passionfruit in Japan.

Nakasato, K., Fujioka, S., Sugawara, Y. et al. First detection of two potyviruses, uraria mosaic virus and passiflora mosaic virus Y, from passionfruit in Japan. J Gen Plant Pathol 86, 401–404 (2020). https://doi.org/10.1007/s10327-020-00932-4

Presentation

Phylogenetic analysis of canine distemper virus lineages circulating in Michigan

October 12, 2024

Poster Presentation at American Association of Veterinary Laboratory Diagnosticians 2024 Annual Conference, Nashville, TN

Deep-sequencing of viruses and viroids in Michigan hop yards: predominance of named ‘latent’ species typically associated with asymptomatic infection

August 01, 2021

Poster Presentation at Plant Health 2021: American Phytopathological Society annual meeting, Online

First detection of two potyviruses from passionfruit in Ogasawara Islands, Japan.

March 01, 2019

Oral Presentation at Annual meeting of the Phytopathological Society of Japan, Tsukuba, Ibaraki, Japan

Kota Nakasato

CV